invisiblemagic🎩

  Aim- To stain nuclear material by Giemsa's method.  Theory- Bacteria do not contain true nucleus. They contain area near the center of the cell. i.e regarded as nuclear structure. These structure has been dedicated nuclear or nucleoid or chromosome. The bacterial nucleoid contain double stranded circular DNA and it can be visible under light microscope by special staining procedure such as Giemsa's method. There are some other method also used of staining of chromatin material which includes feulgens method, rainbow method, acridine orange method etc.  Principle- The demonstration of nucleus require selective hydrolysis of RNA on the cytoplasm with affecting DNA. The acidic nature of bacteria and other organism has affinity towards the basic dyes the hydrolysis of RNA is achieved by acid treatment because of it is double stranded and contain triple bond between guanine and cytosine pair absent in RNA. The acid causes hydrolysis change in confirmation and the aldehyde gr...

Aim-  To detect ability of given micro-organism to produce urease enzyme.                                       Theory-   This test is useful to detect the ability of bacteria to produce urease enzyme. The enzyme is extra cellular induced enzyme. It is specifically act on urea as follows.                                                                                                              Due to hydrolysis basic product is ammonia is produced because of ammonia pH is shifted from acidic to alkaline. In alkaline pH phenol red indicator turns to magenta colour. Then there is urease production indica...