Preparation of media (gelatin agar)

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 Aim- To prepare gelatin agar medium laboratory. 

Theory- Gelatin is protein derive from the animal protein collagen. It is component of connective tissue and tendons gelatin dissolve with water at 50℃ exists as liquid above 25℃. solidify as gets when cool below 25℃ is prepared by boiling of skin and bones gelatin agar medium. It is used as  biochemical test. This medium is to perform gelatin hydrolysis test. In this medium gelatin is rarely use as substrate because it is attached to enzyme and decompose by bacteria nutrient agar is enzyme and decompose by bacteria nutrient agar is use as basal medium KH2PO4 and KH2PO4 is use as buffer and glucose is acts as carbon source.

Procedure- 

1. prepare nutrients agar medium adjust the pH 7.2.                                        

2. After that at gelatin powder heat constant stearing with the boiling of medium.                                                          

3. Media is sterilised by autoclaving.        

4. After cooling it was poured in sterile petry plates and plates where allow to solidify.

Use-                                             

The medium is use to detect ability of given organism to produce gelatinase enzyme. 

Composition-                            

 .Peptone- 1gm.                           

.Nacl-0.5gm.                              

.Meat extract-0.3gm.               

.Agar-Agar- 7.2gm               

.D/W- 100ml.                             

.Gelatin- 4gm.


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