invisiblemagic🎩

  Top 10 richest person in the world  . Jeff bezos   (Net worth $ 202 billion )                                                  Jeff bezos is the chief executive officer (CEO) and founder both of the Amazon. It is a biggest retailer company in the world.  . Elon musk  ( Net worth $177 billion )                                            Elon musk is the (CEO) and Co - founder of tesla. (CEO) He is founder of spacex 🚀  and boring company. He launched 143 satellites 🛰 on one spacecraft. He is also developing planet 🌍. . Bill Gates ( Bet worth $125 billion)                           Bill Gates is a American businessman software developer investor aut...

  Aim- To detect ability of given organism to hydrolyse gelatin or produce gelatinase enzyme or not.  Theory-  Some bacteria have ability to produce extra cellular enzyme like gelatinase. The gelatinase destroy or decompose gelatin.  Reactions- This reaction occurs in two sequential steps.  1. Gelatinase hydrolysis gelatin into polypeptide.  2. That produced polypeptide further converted into amino acids.            That amino acids use by micro-organism for metabolic purpose gelatin hydrolysis test is use in classification and identification of organism. Hydrolysis of gelatin is detected by flooding the plate with Hgcl2. Requirements-  1. Gelatin agar plates.  2. 24hrs old culture of test organism (psuedomonas)  3. Hgcl2. Procedure-  1. A loopful suspension was spot innoculated or sterile gelatin agar plate. 2. The plate was inculated at 37c for 24hrs. 3. After incubation Hgcl2 was poured on the plate and r...

  Aim- To prepare gelatin agar medium laboratory.  Theory- Gelatin is protein derive from the animal protein collagen. It is component of connective tissue and tendons gelatin dissolve with water at 50 ℃ exists as liquid above 25℃. solidify as gets when cool below 25℃ is prepared by boiling of skin and bones gelatin agar medium. It is used as  biochemical test. This medium is to perform gelatin hydrolysis test. In this medium gelatin is rarely use as substrate because it is attached to enzyme and decompose by bacteria nutrient agar is enzyme and decompose by bacteria nutrient agar is use as basal medium KH 2 PO 4  and KH 2 PO 4  is use as buffer and glucose is acts as carbon source. Procedure-   1. prepare nutrients agar medium adjust the pH 7.2.                                         2. After that at gelatin powder heat constant stearing with the ...

  Aim- To stain nuclear material by Giemsa's method.  Theory- Bacteria do not contain true nucleus. They contain area near the center of the cell. i.e regarded as nuclear structure. These structure has been dedicated nuclear or nucleoid or chromosome. The bacterial nucleoid contain double stranded circular DNA and it can be visible under light microscope by special staining procedure such as Giemsa's method. There are some other method also used of staining of chromatin material which includes feulgens method, rainbow method, acridine orange method etc.  Principle- The demonstration of nucleus require selective hydrolysis of RNA on the cytoplasm with affecting DNA. The acidic nature of bacteria and other organism has affinity towards the basic dyes the hydrolysis of RNA is achieved by acid treatment because of it is double stranded and contain triple bond between guanine and cytosine pair absent in RNA. The acid causes hydrolysis change in confirmation and the aldehyde gr...

Aim-  To detect ability of given micro-organism to produce urease enzyme.                                       Theory-   This test is useful to detect the ability of bacteria to produce urease enzyme. The enzyme is extra cellular induced enzyme. It is specifically act on urea as follows.                                                                                                              Due to hydrolysis basic product is ammonia is produced because of ammonia pH is shifted from acidic to alkaline. In alkaline pH phenol red indicator turns to magenta colour. Then there is urease production indica...